FISP Cell-Free Sampling Probe

Flownamics developed the FISP sampling probe in 1996. The FISP sampling probe is capable of withdrawing sterile, cell-free samples from fermenters and bioreactors. FISP allows direct on-line sample transfer to a variety of sampling systems (Seg-Flow), analyzers (such as biochemistry and HPLC systems) and fraction collectors for off-line analysis. FISP is constructed of 316 stainless steel and fitted with a tubular, micro-porous membrane. The probes come in a variety of sizes (6, 12, 19 and 25 mm ports) to accommodate laboratory, pilot or industrial scale vessels. FISP has been and continues to be widely utilized in the biotechnology and bioprocess industries for fermentation and cell-culture since its introduction to the market.

FISP probes can be used for submerged feeding/sterile media addition

• Fast & Easy Setup: Removes the autoclaving reagents step in the process
• Eliminates risk of exposure to hazardous, volatile substances
• 0.2u ceramic membrane provides sterile barrier

Product Specifications

 D-Series FISP Probe

• Available For 19 & 25 mm Ports: Standard and Safety

• Fits Into the Fermenter / Bioreactor Side Port

• Dead Volume: Approximately 0.24 to 0.44 ml

• Used in PD to Production

• GMP Validated

• SIP & CIP Compatible

• Immersion Depths: 90 and 115 mm

• Standard membrane pore size: 0.2 μm

• Stock Membrane Pore Sizes: 0.45, 0.75, 1.0, 5.0, 25.0, & 75.0 μm

• Custom Pore Sizes Available

F-Series FISP Probe

• Available For 8, 12, and 19 mm Ports

• Fits Into the Fermenter / Bioreactor Top Port

• Dead Volume: Approximately 0.24 to 0.44 ml

• Can Be Used With SUBs

• Autoclavable

• Compatible With Kleenpak™ Connectors

• Immersion Depths: 120, 200, 310, and 410 mm

• Standard membrane pore size: 0.2 μm

• Stock Membrane Pore Sizes: 0.45, 0.75, 1.0, 5.0, 25.0, & 75.0 μm

• Custom Pore Sizes Available

Features of the FISP

• Sterile (0.2 μm Filter), Cell-Free Sampling for On-Line or Off-Line Analysis

• Minimal Dead Volume for Consistent and Accurate Sampling, which Reduces Flush Time

• Use with Aerobic or Anaerobic Bacteria, Yeast, Fungi, Algae, Insect, and Mammalian Cell Cultures

• Can Be Used for Submerged Feeding/Sterile Media Addition

• Standard Membrane 0.2 μm

• Additional Stock Pore Sizes: 0.45, 0.75, 1.0, 5.0, 25.0, 75.0 μm (Custom Pore Sizes Available)

Dip Tubes For Full Broth Bioreactor Sampling

For analysis that requires cell-containing samples, Flownamics offers a line of stainless steel probes. These probes, or dip tubes, are autoclavable and can be used with glass, stainless steel, and single use vessels or bags.

Product Specifications

Lengths Available

200, 350, and 450mm

Internal Diameter

0.8mm (0.032 inches)

Outside Diameter

6mm (0.24 inches)

Features & Connections

• Equipped with an adjustable fitting
• Installed into a PG 13.5 port
• Can be adapted to other size ports if needed
• Easy to maintain
• No operator training required
• Used in both fermentation and cell-culture
• Can interface vessels to various sampling systems (Seg-Flow) and analytical equipment
• Minimizes dead volume and purge requirements