Constant High Pressure Cell Disruptor

Constant Brand Introduction：

Constant Systems is a British company specializing in the production of high-pressure cell 

 and is a member of the Score Group PLC group. Constant Systems has been committed to the development and production of high-pressure disruptors, providing users with high-quality and stable performance disruptors. Through its global marketing network, its products are sold to various parts of the world, including North America, Europe, the Middle East, and Asia, providing high-performance high-pressure disruptors and excellent services to pharmaceutical and biotechnology companies, universities, and research institutions worldwide.

Overview of Constant High Pressure Cell Disruptor：

The Constant Systems high-pressure cell disruptor can achieve a maximum pressure of 40000 PSI (2700 bar) and can disrupt microorganisms, animal cells, plant cells, tissues, and homogenization. The Constant Systems high-pressure disruptor has several models including One Shot, TS series, B series, C series, D series, E series, and F series. All models of disruptors can achieve very good disrupting effects after one disrupting. The Constant Systems high-pressure disruptor can conveniently perform CIP (in place cleaning) and SIP (in place disinfection) to meet GMP standards. 

Specific model of Constant cell disruptor：

Constant High Pressure Cell Disruptor   

Domestic assembly models  

Product range

Max Pressure Rating PSI/BAR

FLOW RATE

Oneshot

40KPSI/2700Bar

0.5-8ml

TS0.75KW

40KPSI/2700Bar

40ml/min（2.4L/hr）

TS1.1kw

40KPSI/2700Bar

100ml/min(6L/hr)

Constant High Pressure Cell Disruptor

Original imported models from the UK

Product range

Max Pressure Rating PSI/BAR

FLOW RATE

OS

40KPSI/2700Bar

0.5-8ml

MC

40KPSI/2700Ba

1-40mL

CF1

40KPSI/2700Ba

100ml/min(6L/hr)

CF2

40KPSI/2700Bar

400ml/min（24L/hr）

HFR

40KPSI/2700Bar

150L/hr

The principle of cell fragmentation by a Constant cell disruptor 
The Constant Systems high-pressure cell disruptor is specifically developed for biotechnology users, taking into account the characteristics of biological samples in design. It has unique designs for sample cooling, CIP, and SIP. The principle of the Constant Systems high-pressure disruptor is to use high-pressure extrusion of samples to pass through a fixed small nozzle at high speed, and then collide with the impact target to break the cells.   

The high-pressure piston moves downwards, and due to gravity, the sample is sucked into the high-pressure sleeve

once the sample enters the high-pressure chamber, the piston accelerates at high speed to close the inlet valve and allows the sample to pass through the fixed nozzle at high speed. The sample pressure can reach 40kpsi (2700 bar), and the sample quickly transitions from the high-pressure zone to the low-pressure zone, causing cell fragmentation

The sample hits the impact target and flows through the cooling surface. The cooled sample flows out of the outlet due to gravity and enters the lower cycle

Temperature control                                                                        

By using a cooling jacket to cool the sample during the disrupting process, the temperature of the disrupted sample can be well controlled, thereby avoiding the deactivation of active substances. 

Reproducibility                                               
The Constant Systems fluid control system precisely controls the disrupting pressure, ensuring the reproducibility of the instrument and good disrupting effect. 



Technical characteristics of Constant cell fragmentation equipment

Constant Systems can generate a pressure of up to 40Kpsi (2700 bar), with good disrupting effect. One disrupting can achieve very high disrupting efficiency, avoiding multiple disrupting that may cause fine fragments and denaturation and deactivation of active substances.

Constant Systems disrupting head has a cooling jacket design, which can effectively control the temperature of disrupted samples; Enable the sample to be cooled in a timely manner during the disrupting process, thereby avoiding the deactivation of active substances due to temperature rise in the disrupted sample; In addition, the sample storage tank can also be equipped with a jacket for sample pre cooling.

Constant Systems adopts a fixed nozzle design, which has good stability; Overcoming the disadvantages of valve design that relies on regulating valves to regulate disrupting pressure, such as poor pressure stability, poor reproducibility, and easy valve failure during the disrupting process.

Constant Systems uses hydraulic systems to generate high pressure, with stable pressure and good reproducibility.

The Constant Systems high-pressure cell disruptor has a dead volume of less than 1ml (at least less than 0.1ml) and no minimum sample processing capacity limit.

Constant Systems can process samples with a concentration of up to 30% bacterial concentration, resulting in high concentration of broken sample products, making it convenient for downstream separation and purification operations; Can handle filamentous bacterial cells.

Constant Systems TS, B, and C series optional peristaltic pumps for fully automatic injection and fully enclosed operation, to avoid affecting or being affected by the environment.

The operation and maintenance are simple, and CIP and SIP are very convenient. Specific CIP and SIP designs can be selected, which comply with GMP and FDA standards.

Digital display of operating pressure and sample temperature, and optional data acquisition package to display and record the entire disrupting process's operating pressure and sample temperature, providing useful information for GMP.

Application of Constant Cell Disruptor 
The Constant Systems cell fragmentation instrument is widely used and can be used for cell fragmentation, protein and DNA extraction, enzyme release, DNA manipulation selective fragmentation, single-cell separation, tissue fragmentation, etc., such as: 

 Algae

 disrupting various collar algae

Animal cell

 selection fragmentation of chicken sperm cells

 Microbial

 fragmentation and recombination of Escherichia coli to obtain highly active enzymes; High protein recovery rate achieved by breaking down Mycobacterium

 Fungi (including yeast)

Beer yeast releases 99% of the protein, Pichia pastoris releases 99% of the recombinant protein, and Aspergillus produces high specific activity enzyme

 Mammalian cells and tissues

 Fragmentation of mammalian cells and tissues by horse liver cells and endoplasmic reticulum, isolation of single living cells from bovine spleen and lymph nodes

 insect

 Cytomegalovirus in broken human cell nuclei of insects

 Plant cells and tissues

Extract banana pectin from plant cells and tissues, and extract DNA from strawberry leaves

virus

 Virus isolation of Toxoplasma gondii membrane antigen, separation of amniotic membrane cell nucleus and membrane

 Environmental samples

 Extracting DNA from Soil Microorganisms in Environmental Samples