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Super Electro Cell Fusion Generator ECFG21

Applitech Biological Technology Co., Ltd

Applitech Biological Technology Co., Ltd

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34 Yrs Manufacturer

Price Negotiable

Shipping Notes: Please contact us for more details.

Sample Policy: We offer samples, with sample costs, shipping and taxes paid by the buyer

Product Information

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Origin
Lead Time 15-30 days
Trade Mode * RTS
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Description

Super Electro Cell Fusion Generator ECFG21, the preparation efficiency of monoclonal antibody is much higher than that of PEG method, and it does not depend on the operator. Guaranteed batch stability. The novel three-step electrofusion procedure, based on the conventional electrofusion procedure, with the patented voltage attenuation and polarity alternating pulse (+/-) design, can obtain a higher fusion efficiency and improve cell viability while obtaining a longer repair time after fusion.image.png
  • Step 1:Cells are alignment in alternating current mode
The constant alternating current wave aligns the cells together in a fast two-dimensional electrophoresis. 
  • Step 2:cells are fused in direct current mode
AC/DC switching in a very short time, voltage decay design, can generate forward pulses (+) or polar alternating pulses (+/-). The cells are fused with little damage, and the experimental results show that the pulses with alternating polarity can improve the fusion rate compared with the positive pulses alone.
  • Step 3:Cells are repaired in alternating current mode
Longer cell repair time, optional sine wave with voltage attenuation, stabilize the fusion state of heterozygous cells, promote cell damage repair, and improve survival rate.
Key features:
  • High cell fusion efficiency
  • High cell viability
  • Constant AC voltage and ultra-short AC/DC switching time
  • The new electrofusion program realizes the design of voltage attenuation and polarity alternating pulse (+/-), and the fusion parameters are visible and adjustable
  • Especially suitable for the preparation of monoclonal antibodies, animal cloning and other fields

Applications:Electroporation
Electrofusion
  • Splenocytes are fused with tumor cells for the preparation of monoclonal antibodies
  • Plant protoplast fusion
  • Fusion of mold , yeast

Animal cloning
  • Nuclear transfer, somatic cell nuclear transplantation, somatic cell transfer, somatic cell fusion with egg cell
  • 2-cell blastomeres are electrofused to make tetraploid embryos
  • Activation of mature oocytes in vitro
  • Applicable to a wide range of species, rats, pigs, cats, dogs, wolves, etc

Application example: Miniature pig clone

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Packaging & Logistics

Trade Mode

RTS

Port Not Specified
Lead Time Negotiable
Shipping Terms Contact supplier for shipping details
Payment Terms Negotiable
Currency USD

Payment details are for reference only. Please contact supplier for exact terms.

Business Model Manufacturer
Year Established 1992 (34 years)
Number of Employees 100-499人
Location China

Company Introduction

Applitech Biological Technology Co., Ltd., as a high-tech enterprise integrating design, R & D, production, sales and service, we are committed to providing professional production and analysis equipments, single-use consumables and comprehensive solutions for the biopharmaceutical field. Since our establishment, we have always been custom er-centered and taken "Quality First, Service Foremost" as our business principle. Based on the optimization, scale-up and production of bioprocess, we have been continuously improving the product lines in the biopharmaceutical field, providing users with all-round professional solutions for biotechnology and helping them to continuously make new breakthroughs in the biopharmaceutical field.


With nearly two decades of expertise in the bioreactor field, Applitech is dedicated to developing premium, self-brand ed bioreactors. Our product range spans from R&D to pilot-scale and cGMP production, with applications in antibody, vaccine, cell therapy, gene therapy, and stem cell therapy R&D and manufacturing. Centered on bioreactor technolo gy, we have established a comprehensive product ecosystem encompassing process monitoring, spectroscopic analysis, cell analysis, online sampling, sample treatment, and etc., providing a extensive product portfolio and technical support for domestic and international biopharmaceutical companies.


Applitech is headquartered in Guangzhou,with sales and after-sales service network covering the entire market in China. We have established branches and product exhibition and application centers in Shanghai and Beijing, offices in Chengdu and Xi'an, and service sites in Tianjin, Suzhou, Hangzhou, Wuhan, Nanjing, Lanzhou, Changchun, Qingdao, etc. Driven by our commitment to “technical expertise, considerate service and rapid response”, Applitech is customer-oriented, and collaborates closely with its technology partners to safeguard the progress and development of the biopharmaceutical industry!

Contact Information

Applitech Biological Technology Co., Ltd

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Carrie

Address

No. 483 XingNan Road,Panyu District,Guangzhou, China

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