Our new, highly efficient and gentle single-cell dispenser with fluorescent sorting. Streamline your workflows from monoclonal cell culture development for biopharmaceutical manufacturing, to single-cell assays for basic scientific research.

Building on the previous generation of single-cell dispensers, the F.SIGHT 2.0 has an innovative and ultrafast dual-camera system for simultaneous brightfield and fluorescent full resolution images.

Key Features & Benefits

Fast and accurate single-cell dispenser

Single cells are gently dispensed into a 96-well plate in under 2 minutes or into a 384-well plate in under 8.

Dual imaging system

Overlay both brightfield and fluorescent images for precise identification of your cells of interest. Both cameras can be controlled independently and the power of the built-in blue laser is adjustable. Combined, their high dynamic range visualizes even the weakest fluorophores.

No risk of contamination

Eliminate the risk of cross contamination with single-use EASY.ON cartridges.

Assurance of single-cell isolation

Capture nozzle images for every dispensing run to confirm that only single cells were dispensed on the plate.

Cell Focusing technology

Align cells at the center of the dispensing cartridge for even better handling and processing of delicate samples.

Gentle single-cell dispensing

Handle and isolate delicate cells of interest with our no-pressure dispensing, minimizing the stress inflicted on cells.

Product Details

Fast and precise dispensing

The F.SIGHT 2.0 isolates single-cells in either 96- or 384-well plates quickly, dispensing an entire 384-well plate in under 8 minutes. The system’s versatility enables a multitude of different cells to be fluorescently labelled and isolated.

Minimizing cell loss

Add an additional layer of optimization with Cell Focusing technology, which gently aligns cells at the center of the dispensing cartridge for superior cell detection. It offers significant improvements when working with rare cell types as this alignment ensures that cells are perfectly captured and measured, reducing cell loss and even increasing processing speeds.

Powerful software

Following CYTENA’s customer-oriented philosophy, the software has been designed for easy use and quick setup of individual fluorescence experiments. Additionally, the F.SIGHT 2.0’s software can provide full resolution brightfield and fluorescence images along with an overlayed image for monoclonality assurance. Everything is always recorded and saved for future analysis and assurance of clonality.

Automation ready

The F.SIGHT 2.0 can be easily integrated into automated workflows, making it the fastest single-cell dispenser fully compatible with robotic automation.

Product Applications

Cell line development

Gene therapy and viral vector production

Cell therapy and stem cell-based research

CRISPR gene editing

Exosome manufacturing

Featured Workflow

With the F.SIGHT 2.0’s fluorescet sorting capabilities, only single cells of interest are isolated and dispensed for monoclonal expansion. After isolation, clones of interest are expanded and, subsequently, the clones with the best yield are selected.

Technical Specifications

Samples          

Eukaryotic cells

Cell size        

5 – 40 µm

Sorting           

Cell morphology and fluorescence

Droplet volume

~200 pL

Substrate

96- and 384-well plates

Nozzle imaging

Camera CMOS

Magnification 10x

Dual-channel: Brightfield and fluorescence

Fluorescence nozzle imaging

Ex. 488 nm

Em. 520 +/- 36 nm

Processing times

Single-cell dispensing 96-well plate: ~ 2 min

Single-cell dispensing 384-well plate: ~ 8 min

Cartridge

EASY.ON 40 µm disposable cartridge to avoid cross-contamination

Automation

Lid opening and closing

Compatible with standard automation arm and gripper

Compatible with third-party automation clients

Embedded computer

Windows

Compatibility

Standard biosafety cabinets class 2

Footprint

635 x 400 x 286 mm

Weight

40 kg

Certification

CE, CB, UL (TÜV), RoHS