AbioBundle Series Glass Bioreactors

With nearly 20 years of experience in the biopharmceutical industry and a dedication to craft high-end bioreactors, Applitech proudly presents the AbioBundle Series Glass Bioreactors. Backed by a highly skilled R&D team of over 150 professionals, the AbioBundle line represents years of refinement and expertise.

Designed with a deep understanding of user needs, AbioBundle M/E/P/D series bioreactors set new standards in process development. With AbioBundle, Applitech provides unparalleled support for your bioprocess development.

1.1 AbioBundle M-Series Bioreactors

The AbioBundle M Series Bioreactors feature a classic small-scale bioreactor design, enabling precise downscaling of laboratory-scale reactors while maintaining the same flexibility and adaptability. The system offers customizable configurations to meet diverse customer needs.

m-Control

Features:

• The working volume covers 50ml~2.4L.
• Embedded m-Control offers multi-functionality in a small footprint; customizable casing in multiple colors.
• Parallel cultivation with independent monitoring of each reactor.
• Up to 32 m-Control controllers form a network of parallel reactors.
• Control operations easily through web browsers or mobile devices (data can be stored and monitored without SCADA software).
• Data can be scaled up directly, minimizing media consumption and saving cost.
• 250mL/500mL small bioreactors use a Peltier-based temperature control system, eliminating the need for a chiller.
• Small footprint, less than 1m² for a 4-tank system.
• online automatic detection of control parameters: pH, temperature, DO, agitation, level, foam, etc.
• Modular design allows easy adjustments to suit various applications and process control strategies.
• AiOpt algorithm for precise control of process parameters.
• Up to 4 variable-speed pumps, each with an independent Prime button.
• Up to 5 gas mass flow controllers (MFCs).
• Supports commonly used electrodes in the market and is compatible with smart electrodes.
• Total Gas Flow Control
• The aeration system includes 1 overlay and 2 sparger ports, with options for different sparger sizes and shapes.
• The agitation system features a servo motor and various impeller types, suitable for cell culture or microbial fermentation.
• Equipped with a unique breathing light to indicate system working status.
• Compatible with external devices such as cell density modules, fluorescent pH/DO electrodes, balances, etc.
• Comes standard with AbioPIMS Lite or AbioExpert Lite software.
• (Optional) Configurable to support vessel sizes up to 15L.
• (Optional) Enables remote monitoring capabilities.
• (Optional) Provides remote alarm notifications via SMS, phone calls, email or WeChat.
• (Optional) Advanced functionalities such as enhanced feeding strategies tailored to customer needs.

Applications

• Mammalian and stem cell, microbial, fungal, plant cell, insect cell culture Cell line screening, process optimization, media optimization, and toxicity studies.
• High-density, aerobic, and anaerobic fermentation; protein expression research.
• Process development for vaccines, antibodies, and cell/gene therapies.
• Batch, fed-batch, perfusion, and continuous culture.
• R&D, Pilot and small-scale production work.

Size & Model

1.2 AbioBundle E-Series Bioreactors

The AbioBundle E Series Bioreactors are robust, flexible, and easy to operate, with options for upgrades and expansions. Ideal for mammalian cell culture and microbial fermentation R&D projects, they offer configurations for single, dual, or quad controls. Supporting both single-wall and jacketed vessels, the series meets the demands of complex processes and is suited for a wide range of applications. The dual/quad control modes allow for mixing and matching of vessels with different volumes and types, all operating independently.

ePlus-Control

eDual -Control

Features:

• The working volume covers 0.3~16L

• Modular Design: Add or remove functional modules based on application requirements.

• No welded components on the head plate; all metal parts are made from 316L stainless steel. Non-contact areas have a mirror-polished finish, and contact areas are electro-polished for easy cleaning.

• Borosilicate Glass Vessels offer excellent resistance to shock and corrosion with smooth surfaces for easy cleaning.

• Available with single-control (ePlus-Control), dual-control (eDual-Control, supports quad-control).

• 13.3” touchscreen and colorful interface for intuitive operation.

• Built-in storage to ensure no data loss.

• Supports up to 23 operator accounts simultaneously with three-level password protection.

• Features a web interface for easy bioreactor setup and control

• via a browser.

• (Optional) Biomass module enables real-time cell density measurement and feedback feeding operations.

• Agitation system: Equipped with a servo motor and mechanical seal stirrer; upgradeable to magnetic coupling.

• (Optional) Enhanced gas module allows switching between 1 overlay and 2 sparger pathways per gas.

• Each vessel supports up to 6 mass flow controllers (MFCs).

• Feeding control: Supports external balance integration for advanced feeding strategies via PID control.

• ePlus-Control supports up to 4 constant-speed pumps, with optional variable-speed pumps. eDual-Control supports up to 4 variable-speed pumps per vessel, each with an independent mechanical Prime button.

• Total Gas Flow Control: Maintains constant total gas flow during culture to prevent excessive shear forces on cells.

• Comes standard with AbioPIMS Lite or AbioExpert Lite software.

• (Optional) Enables remote monitoring capabilities.

• (Optional) Provides remote alarm notifications via SMS, phone calls, email or WeChat.

• (Optional) Advanced functionalities such as enhanced feeding strategies tailored to customer needs

Applications

• Mammalian and stem cell, microbial, fungal, plant cell, insect cell culture.
• Cell line screening, process optimization, media optimization, and toxicity studies.
• High-density, aerobic, and anaerobic fermentation; protein expression research.
• Process development for vaccines, antibodies, and cell/gene therapies.
• Batch, fed-batch, perfusion, and continuous culture.
• R&D, Pilot and small-scale production work.

Size & Model

1.3 AbioBundle P-Series Bioreactors

The P Series Bioreactors utilize a PLC-based control system designed for ease of use. Suitable for applications ranging from laboratory research to pilot-scale and production-scale operations, the system minimizes time and cost associated with scaling up. The p-Control controller can manage multiple vessels simultaneously, with options for tailored expansion.

p-Control

Features:

• The working volume covers 0.3~16L.

• Touchscreen HMI, intuitive and user-friendly interface for simplified operation.

• Siemens PLC platform.

• Standard Fieldbus I/O Design to failitate easy maintenance and functional expansion.

• Flexibility & Scalability, providing superior reliability and adaptability.

• Open plateform that supports integration with systems like Pi data recording.

• Compliant with ISAS88, GAMP5, cGMP, etc.

• Measurement and Control Parameters:

  - pH, dissolved oxygen, temperature, foam, level, agitation.

  - Up to 6 gas channels per vessel.

  - Balance

  - Up to 4 built-in variable-speed pumps per vessel

• Enables simultaneous control of multiple bioreactors with different sizes, making scale-up more efficient.

• Comes standard with AbioPIMS Lite or AbioExpert Lite software.

Application

• GMP-compliant with audit trail for R&D or small-scale production.
• Mammalian and stem cell, microbial, fungal, plant cell, insect cell culture.
• Cell line screening, process optimization, media optimization, and toxicity studies.
• High-density, aerobic, and anaerobic fermentation; protein expression research.
• Process development for vaccines, antibodies, and cell/gene therapies.
• Batch, fed-batch, perfusion, and continuous culture.
• R&D, Pilot and small-scale production work.

2. AppliSoftec Data Management Software

AppliSoftec, consisting of the AbioPIMS and AbioExpert series, is Applitech’s proprietary bioprocess information management system.

AbioPIMS/AbioExpert complies with GAMP5, 21 CFR Part 11, and ANSI/ISA-88.01 Batch Control System Standards, offering robust audit trails. The software facilitates real-time data acquisition of bioprocess parameters, storing data in database for historical data analysis and traceability, and empowering enhanced production, quality, and equipment management.

AbioPIMS/AbioExpert is compatible with OPC UA protocol. AbioExpert can integrate with external devices (such as Nova Biochemical Analyzer, Kaiser Raman Analyzer, and cell counters) for advanced process strategies.

AbioPIMS/AbioExpert has a RECIPE design functionality with a graphical programming interface. Users can implement complex process strategies without any programming expertise.

AbioPIMS/AbioExpert is compatible with Applikon my-Control/ez-Control/ez2-Control series bioreactors.

AppliSoftec Bioprocess Information Management System

AppliSoftec is available in two series: AbioPIMS and AbioExpert, with each series divided into four versions based on functionality: Lite, Standard, Advanced, and GMP. AbioPIMS series supports AbioSUS Single-Use Bioreactors, AbioWave Single-Use Rocking Bioreactors, and AbioBundle M/E/P Series Glass Bioreac tors. AbioExpert Series supports AbioBundle M/E/P Series Bioreactors, AbioPilot Stainless Steel Bioreactors, and can also collect data from analytical devices such as Nova and Kaiser.The AppliSoftec Series Software is also compatible with the Applikon my-Con trol, ez-Control, and ez2-Control series bioreactors.
The AbioBundle M/E/P Series Bioreactors come with AbioPIMS Lite or AbioEx pert Lite Software by default, with optional upgrades to higher-level versions within the same series.

Features

• Enables enterprise-wide data management and information sharing across all divisions and subsidiaries. • Rapidly addresses the needs of bioprocess development and production. • Enhances efficiency, cost-effectiveness, and reduces engineering expens es. • Compatible with major bioreactor platforms, integrating seamlessly with analytical and operational instruments for comprehensive data acquisition.